Journal: Biomolecules

Article Title: Evaluation of Plasma-Derived hsa_circ_003077 for Non-Invasive Diagnosis of Alzheimer’s Disease

doi: 10.3390/biom16030356

Figure Lengend Snippet: Plasma glial and receptor biomarkers in Alzheimer’s disease (AD) patients and cognitively healthy controls (HC). ( a ) GFAP, ( b ) NfL, ( c ) Tyro3, and ( d ) AXL levels were measured in plasma. Data are presented as mean ± SEM. Statistical comparisons were performed using the Mann–Whitney U test. Statistical significance is indicated as ** p < 0.01; *** p < 0.001; ns, not significant.

Article Snippet: The Human Tyro3 DuoSet ELISA kit (Catalog No: DY8596-05) supplied by R&D Systems (Minneapolis, MN, USA) was used to measure Tyro3 levels, while the Human AXL DuoSet ELISA kit (Catalog No: DAXL00; R&D Systems, Minneapolis, MN, USA) was used to measure AXL levels.

Techniques: Clinical Proteomics, MANN-WHITNEY

Journal: Biomolecules

Article Title: Evaluation of Plasma-Derived hsa_circ_003077 for Non-Invasive Diagnosis of Alzheimer’s Disease

doi: 10.3390/biom16030356

Figure Lengend Snippet: Diagnostic performance of plasma biomarkers for differentiating Alzheimer’s disease (AD) from cognitively healthy controls (HC) based on ROC curve analysis. Receiver operating characteristic (ROC) curves were generated to assess the diagnostic accuracy of plasma biomarkers. The area under the ROC curve (AUC) was used to evaluate biomarker performance: 0.90–1.00, superior diagnostic efficacy; 0.80–0.89, good diagnostic efficacy; 0.70–0.79, moderate diagnostic efficacy; <0.70, poor/substandard diagnostic efficacy. Data are presented as mean ± SEM. Statistical comparisons were performed using the Mann–Whitney U test. hsa_circ_003077 exhibited the highest diagnostic accuracy (AUC = 0.90; 95% CI: 0.82–0.97), followed by NfL (AUC = 0.75) and pTau-217 (AUC = 0.77). AXL and GFAP showed lower diagnostic performance (AUC = 0.63 and 0.69, respectively), while pTau-181 and Tyro3 demonstrated substandard efficacy (AUC = 0.63 each).

Article Snippet: The Human Tyro3 DuoSet ELISA kit (Catalog No: DY8596-05) supplied by R&D Systems (Minneapolis, MN, USA) was used to measure Tyro3 levels, while the Human AXL DuoSet ELISA kit (Catalog No: DAXL00; R&D Systems, Minneapolis, MN, USA) was used to measure AXL levels.

Techniques: Diagnostic Assay, Clinical Proteomics, Generated, Biomarker Discovery, MANN-WHITNEY

Journal: NPJ Vaccines

Article Title: Protective efficacy of the pan-fungal vaccine NXT-2 against vulvovaginal candidiasis in a murine model

doi: 10.1038/s41541-025-01171-4

Figure Lengend Snippet: IL-1β ( A ) and S100A8 ( B ) concentrations were assessed via ELISA. All data represents mean ± SEM. Statistical significance was assessed by using the Mann-Whitney test for IL-1β and S100A8 concentrations. C Tissue damage was assessed by measuring lactate dehydrogenase (LDH) activity in vaginal lavage fluid twenty-eight days post vaccination (28dpv) and five days post challenge (5dpc). Data represents mean ± SEM. Statistical significance was assessed by two-way ANOVA with Tukey correction (* P = 0.0111, *** P = 0.0007, **** P < 0.0001). D Quantification of inflammation in vaginal tissue from sham and NXT-2 immunized mice excised 5 days post challenge. Data represents mean ± SEM. Statistical significance was assessed by unpaired student t -test (**** P < 0.0001). Vaginal tissue excised following immunization and C. albicans challenge was stained with H&E (sham— E , NXT-2— G ) and the neutrophil specific marker anti-mouse Ly6G (sham— F , NXT-2— H ). Tissue sections were scanned at 40× objective magnification using the Aperio AT2 (Leica Biosystems). Image analysis and capture was performed using ImageScope (Leica Biosystems) at 20× digital zoom level.

Article Snippet: S100A8 : Utilizing the Mouse S100A8 ELISA kit (Rockland Immunochemicals), samples were diluted 1:100 and the manufacturer’s instructions were followed.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Activity Assay, Staining, Marker

Journal: NPJ Vaccines

Article Title: Protective efficacy of the pan-fungal vaccine NXT-2 against vulvovaginal candidiasis in a murine model

doi: 10.1038/s41541-025-01171-4

Figure Lengend Snippet: IL-1β ( A ) and S100A8 ( B ) concentrations were assessed via ELISA. All data represents mean ± SEM. Statistical significance was assessed by using the Mann-Whitney test for IL-1β and S100A8 concentrations. C Tissue damage was assessed by measuring lactate dehydrogenase (LDH) activity in vaginal lavage fluid twenty-eight days post vaccination (28dpv) and five days post challenge (5dpc). Data represents mean ± SEM. Statistical significance was assessed by two-way ANOVA with Tukey correction (* P = 0.0111, *** P = 0.0007, **** P < 0.0001). D Quantification of inflammation in vaginal tissue from sham and NXT-2 immunized mice excised 5 days post challenge. Data represents mean ± SEM. Statistical significance was assessed by unpaired student t -test (**** P < 0.0001). Vaginal tissue excised following immunization and C. albicans challenge was stained with H&E (sham— E , NXT-2— G ) and the neutrophil specific marker anti-mouse Ly6G (sham— F , NXT-2— H ). Tissue sections were scanned at 40× objective magnification using the Aperio AT2 (Leica Biosystems). Image analysis and capture was performed using ImageScope (Leica Biosystems) at 20× digital zoom level.

Article Snippet: S100A8 : Utilizing the Mouse S100A8 ELISA kit (Rockland Immunochemicals), samples were diluted 1:100 and the manufacturer’s instructions were followed.

Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY, Activity Assay, Staining, Marker